Measuring containment of viable infectious cell sorting in high-velocity cell sorters

Measuring containment of viable infectious cell sorting in high-velocity cell sorters.

BACKGROUND With the advent of high-speed sorters, aerosols are a considerable safety concern when sorting viable infectious materials. We describe a four-part safety procedure for validating the containment. METHODS This procedure includes aerosol containment, physical barriers, environmental controls, and personal protection. The Aerosol Management System (AMS) produces a negative pressure w...

Constructing Sorting Networks from k-Sorters

We study the problem of using sorters of k vabxes to form large sorting and merging networks. For n an integral power of k, we show how to merge k sorted vectors of length n/k each using 4 log, n 3 layers of k-sorters and 4 log* n 5 layers of k=ittiqut binary mergers. As a result, we show how to sort R v&es using 2 iag$ n -log, n layers of k-sorters and 2 logf n 3 fog& n * I fayers of k-input l...

Elastomeric Microfabricated Fluorescence-Activated Cell Sorters (μFACS)

Epithelial polarity: Sorting out the sorters

Epithelial cell polarity depends on the continuous sorting of plasma membrane proteins. While various sorting signals and pathways have been identified, only recently has a protein been identified that recognizes such sorting determinants and mediates sorting to a specific cell-surface domain.

Standard safety practices for sorting of unfixed cells.

Cell sorting of viable biological specimens has become widespread in laboratories involved in basic and clinical research. As these samples can contain infectious agents, precautions to protect instrument operators and the environment from hazards arising from the use of sorters are paramount. This unit presents a revised and updated version of the biosafety guidelines for sorting of unfixed ce...